Synergistic processing of biphenyl and benzoate: Carbon flow through the bacterial community in polychlorinated-biphenyl-contaminated soil

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901733" target="_blank" >RIV/60461373:22330/16:43901733 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/srep22145" target="_blank" >http://dx.doi.org/10.1038/srep22145</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep22145" target="_blank" >10.1038/srep22145</a>

Result language

angličtina

Original language name

Synergistic processing of biphenyl and benzoate: Carbon flow through the bacterial community in polychlorinated-biphenyl-contaminated soil

Original language description

Polychlorinated biphenyls (PCBs) are toxic and persistent organic pollutants that can be transformed to varying extents by some microorganisms in soil. The aerobic mineralization of PCBs involves the upper (biphenyl, BP) and lower (benzoate, BZ) pathways, but interactions between members of the microbial community active throughout the stages of PCB biodegradation are not well understood. This study investigates BP and BZ biodegradation and subsequent carbon flow between these and other guilds in PCB-contaminated soil from Kodiak, Alaska. DNA stable isotope probing (SIP) was used to identify members of the bacterial community involved in utilization of 13C-biphenyl (an analogue of PCBs), and 13C-benzoate (a product/intermediate of biphenyl degradation and analogue of chlorobenzoates). By performing SIP in parallel with these two substrates over a time course, we reveal microbes performing the upper (BP) and/or lower (BZ) degradation pathways, as well as heterotrophic bacteria involved indirectly in processing of carbon derived from these substrates. Different members of the phyla Actinobacteria and Proteobacteria dominated the biotransformation of BP and BZ in soil. Substrate mineralization rates and shifts in relative abundance and phylogenetic identity of labeled organisms suggest that BP and BZ biotransformations were performed by microorganisms with different growth strategies: BZ-associated bacteria were fast growing, potentially copiotrophic organisms, while microbes that transform BP were oliotrophic, slower growing, organisms. Overall, our findings provide novel insight into the functional interactions of soil bacterial community members active in processing biphenyl and related aromatic compounds in soil, and reveal how carbon flows through a bacterial community.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EH - Ecology - communities

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

FEB 26 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

22145

UT code for WoS article

000370864100004

EID of the result in the Scopus database

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