New findings in potential applications of tobacco osmotin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43902015" target="_blank" >RIV/60461373:22330/16:43902015 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.pep.2016.09.008" target="_blank" >http://dx.doi.org/10.1016/j.pep.2016.09.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2016.09.008" target="_blank" >10.1016/j.pep.2016.09.008</a>

Result language
angličtina
Original language name
New findings in potential applications of tobacco osmotin
Original language description
The osmotin protein is involved in both monocot and dicot plant responses to biotic and abiotic stress. To determine the biological activity of osmotin, the gene was amplified from tobacco genomic DNA, fused with the hexahistidine tag motif and successfully expressed in Escherichia coli, after which the recombinant osmotin was purified and renatured. Various activities were then tested, including hemolytic activity, toxicity against human embryonic kidney cells, and the antifungal activity of the recombinant osmotin. We found that osmotin had no adverse effects on human kidney cells up to a concentration of 500 mu g.ml(-1). However, the purified osmotin also had significant antimicrobial activity, specifically against fungal pathogens causing candidiasis and otitis, and against the common food pathogens.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GA15-22276S" target="_blank" >GA15-22276S: Preparation of transgenic plants with increased stress resistance</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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