ANALYSIS OF CLOSTRIDIUM BEIJERINCKII NRRL B-598 CODING REGIONS USING RNA-SEQ DATA OF A CLOSELY RELATED STRAIN

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43913810" target="_blank" >RIV/60461373:22330/17:43913810 - isvavai.cz</a>

Result on the web

<a href="http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf" target="_blank" >http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

ANALYSIS OF CLOSTRIDIUM BEIJERINCKII NRRL B-598 CODING REGIONS USING RNA-SEQ DATA OF A CLOSELY RELATED STRAIN

Original language description

Modern research in biotechnology utilizes methods of molecular biology and associated bioinformatics techniques more than in the past. Genome mining of biotechnologically relevant organisms became a standard procedure. After a genome is sequenced and annotated, more information regarding its genes, including their variant calling, and the analysis of their expression needs to be acquired. This can be achieved by additional sequencing of the transcriptome, so-called RNA-Seq. Here, we present the analysis of the coding regions for a recently reidentified strain Clostridium beijerinckii NRRL B-598, formerly misidentified as C. pasteurianum, using RNA-Seq expression data of a closely related strain C. beijerinckii NCIMB 8052. We confirm the correctness of its reidentification as the majority of reads match the genome. Although expression levels or single nucleotide variants cannot be properly explored, we are able to analyze in silico the annotation of this genome, which supports the annotation of biotechnologically relevant genes. Although this kind of analysis does not allow us to reannotate the genome, nor to reconstruct any gene regulatory networks, it still provides us with valuable information for planning our own RNA-Seq experiments that will be performed in the near future.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 5th International Conference on Chemical Technology

ISBN

978-80-86238-65-4

ISSN

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e-ISSN

neuvedeno

Number of pages

5

Pages from-to

87-91

Publisher name

Česká společnost průmyslové chemie (ČSPCH)

Place of publication

Praha

Event location

Mikulov

Event date

Apr 10, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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