PUX10 associates with CDC48A and regulates the dislocation of ubiquitinated oleosins from seed lipid droplets

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43915971" target="_blank" >RIV/60461373:22330/18:43915971 - isvavai.cz</a>

Result on the web

<a href="http://www.plantcell.org/content/30/9/2116" target="_blank" >http://www.plantcell.org/content/30/9/2116</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1105/tpc.18.00275" target="_blank" >10.1105/tpc.18.00275</a>

Result language

angličtina

Original language name

PUX10 associates with CDC48A and regulates the dislocation of ubiquitinated oleosins from seed lipid droplets

Original language description

Postgerminative mobilization of neutral lipids stored in seed lipid droplets (LDs) is preceded by the degradation of oleosins, the major structural LD proteins that stabilize LDs in dry seeds. We previously showed that Arabidopsis thaliana oleosins are marked for degradation by ubiquitination and are extracted from LDs before proteolysis. However, the mechanisms underlying the dislocation of these LD-anchored proteins from the LD monolayer are yet unknown. Here, we report that PUX10, a member of the plant UBX-domain containing (PUX) protein family, is an integral LD protein that associates with a subpopulation of LDs during seed germination. In pux10 mutant seedlings, PUX10 deficiency impaired the degradation of ubiquitinated oleosins and prevented the extraction of ubiquitinated oleosins from LDs. We also showed that PUX10 interacts with ubiquitin and CDC48A, the AAA ATPase Cell Division Cycle 48, through its UBA and UBX domains, respectively. Collectively, these results strongly suggest that PUX10 is an adaptor recruiting CDC48A to ubiquitinated oleosins, thus facilitating the dislocation of oleosins from LDs by the segregase activity of CDC48A. We propose that PUX10 and CDC48A are core components of a LD-associated degradation machinery, which we named the LD-associated degradation system. Importantly, PUX10 is also the first determinant of a LD subpopulation described in plants, suggesting functional differentiation of LDs in Arabidopsis seedlings.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell

ISSN

1040-4651

e-ISSN

—

Volume of the periodical

30

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

22

Pages from-to

2116-2136

UT code for WoS article

000446563700014

EID of the result in the Scopus database

2-s2.0-85054774483