Biophysical study of CDTE quantum dots interactions with albumin and antibody as the base of photodynamic therapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43916161" target="_blank" >RIV/60461373:22330/18:43916161 - isvavai.cz</a>

Result on the web

<a href="https://www.nanocon.eu/files/uploads/01/NANOCON2017_Proceedings_content.pdf" target="_blank" >https://www.nanocon.eu/files/uploads/01/NANOCON2017_Proceedings_content.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Biophysical study of CDTE quantum dots interactions with albumin and antibody as the base of photodynamic therapy

Original language description

Photodynamic therapy is a relatively new type of cancer treatment, or eventually it is used at the elimination of undesirable pathogenic microorganisms. Quantum dots (QDs) are semiconducting nanocrystals with a size of 2-20 nm and can be used in photodynamic therapy. Two types of CdTe QDs were prepared by microwave synthesis (500 W), typical absorption spectra had maxima lambda(green) = 554 nm, lambda(yellow) = 580 nm. The determined size of the generated nanoparticles ranged between 5 -10 nm. CdTe QDs were further studied by fluorescence analysis at excitation wavelength lambda = 250 nm to obtain emission maxima (lambda(em552)) for green and (lambda(em582)) for yellow nanoparticles. Interaction study of CdTe QDs with bovine serum albumin (BSA) and with polyclonal chicken antibodies against sarcosine (AntiSar) was performed. BSA (100, 50, 25, 12.5, 6.3, 3.1, 1.6 and 0 mu M) and AntiSar (20, 10, 5, 2.5, 1.3, 0.7, 0.3, and 0 g/L) were monitored with 1:1 addition of 50 mu M QDs(green) or QDs(yellow). The decrease in intensity of the normalized fluorescence signal in the presence of BSA or AntiSar was observed by 70-90%. The observed dependencies showed a linear trend (R-2 = 0.9) with relative error of 9-12% calculated from 5 independent repetitions. In addition, it was possible to monitor the signal shift to shorter wavelengths at the highest applied BSA concentration by 4-16 nm. The obtained data suggest that the size of individual QDs will affect intensity of an interaction with biomolecule. Nanoconstructs should therefore be targeted according to these experimental data for their intended use. In further experiments, CdTe QDs modified with AntiSar will be used for targeted prostate cancer therapy using photodynamic effect.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Nanocon 2017

ISBN

978-80-87294-81-9

ISSN

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e-ISSN

neuvedeno

Number of pages

6

Pages from-to

537-542

Publisher name

Tanger s.r.o.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 18, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000452823300088