TOLERANCE LEVELS TO SUBSTRATES OF EFFLUX PUMPS IN CLOSTRIDIUM BEIJERINCKII NRRL B-598 AND ITS BUTANOL RESISTANT MUTANTS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43916808" target="_blank" >RIV/60461373:22330/18:43916808 - isvavai.cz</a>

Result on the web

<a href="https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf" target="_blank" >https://www.icct.cz/AngiologyKlon-ICCT/media/system/2018/ICCT-2018-Proceedings.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

TOLERANCE LEVELS TO SUBSTRATES OF EFFLUX PUMPS IN CLOSTRIDIUM BEIJERINCKII NRRL B-598 AND ITS BUTANOL RESISTANT MUTANTS

Original language description

Butanol with its excellent physical and chemical properties is a perspective biofuel and gasoline additive. Its production by Clostridium species using ABE fermentation is an attractive and promising, however, not profitable process mostly due to the low titer produced. This bottleneck is caused by high toxicity of butanol to the production cells and one of the most promising mechanisms, that can be used to overcome it, is increase in expression of efflux pump genes which may be followed by increased formation of efflux pump proteins. As this would lead to the increase in active export of produced butanol to the fermentation medium, bacterial cells can obtain improved resistance and subsequently improved production. As not much knowledge is yet available about efflux mechanisms in Clostridia, our aim was to examine tolerance levels to different known substrates of efflux pumps relevant for the production strain Clostridium beijerinckiiNRRL B-598 and its mutant strains with increased butanol resistance. Mutant strains were obtained either by random chemical mutagenesis followed by selection on a TYA agar medium containing butanol or ethidium bromide EtBr (known efflux inducer) in a lethal concentration of both compounds for the parental strain or by direct cultivation of the parental strain on the agar medium containing EtBr . The broth dilution method was used for the determination of inhibitory concentrations of butanol, ethanol, ethidium bromide and five antibiotics (ampicillin, chloramphenicol, erythromycin, tetracycline and streptomycin). Tolerance to these substrates was tested in 96-well microtiter plate filled with TYA medium and acid base indicator bromocresol purple for the visual control of growth.Obtained results contribute to better understanding of efflux mechanisms in Clostridia, and it is possible that increase of butanol tolerance may be caused by enhancement of the activity of previously unknown type of efflux pump capable of specific butanol excretion.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

PROCEEDINGS of the 6th International Conference on Chemical Technology

ISBN

978-80-86238-77-7

ISSN

2336-8128

e-ISSN

neuvedeno

Number of pages

6

Pages from-to

21-26

Publisher name

Česká společnost průmyslové chemie (ČSPCH)

Place of publication

Praha

Event location

Mikulov

Event date

Apr 16, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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