Comparison of methods for identification of microbial communities in book collections: Culture-dependent (sequencing and MALDI-TOF MS) and culture-independent (Illumina MiSeq)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43917530" target="_blank" >RIV/60461373:22330/18:43917530 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22810/18:43917530
Result on the web
<a href="https://www-sciencedirect-com.ezproxy.vscht.cz/science/article/pii/S0964830516306254?via%3Dihub" target="_blank" >https://www-sciencedirect-com.ezproxy.vscht.cz/science/article/pii/S0964830516306254?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ibiod.2017.02.015" target="_blank" >10.1016/j.ibiod.2017.02.015</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of methods for identification of microbial communities in book collections: Culture-dependent (sequencing and MALDI-TOF MS) and culture-independent (Illumina MiSeq)
Original language description
Different identification strategies employing new methodologies, namely, culture-dependent (using matrix-assisted laser desorption/ionization – time-of-flight mass spectrometry, MALDI-TOF MS) and culture-independent (using high-throughput sequencing on Illumina MiSeq platform) approaches, were applied for the first time to analysis of the deteriorating microflora of book samples. We compared two different identification techniques coupled to microbial cultivation: DNA sequencing and MALDI-TOF MS. The two identification systems produced almost the same results. DNA sequencing was able to better identify all the recovered isolates, while MALDI-TOF MS failed to properly recognize Streptomyces ambofaciens and Lysinibacillus fusiformis, the latter method permitting identification at least at genus level. In addition, the isolates Myxotrichum deflexum and Oidiodendron cerealis were not identified by the MALDI-TOF MS approach. This incongruence was caused by the absence of different kinds of microorganisms in the MALDI BioTyper reference database. The Illumina high-throughput sequencing coupled to non-invasive sampling allowed the identification of a complex bacterial and fungal community. The study presents the satisfactory performance of two novel alternative identification methods (MALDI-TOF MS and Illumina MiSeq) for the investigation of microflora colonizing archival items. © 2017 Elsevier Ltd
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Biodeterioration and Biodegradation
ISSN
0964-8305
e-ISSN
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Volume of the periodical
131
Issue of the periodical within the volume
JULY 2018
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
51-59
UT code for WoS article
000438480000007
EID of the result in the Scopus database
2-s2.0-85015397293