Selection of Bacterial Strain for Lactic Acid Production from Straw Hydrolysate

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918838" target="_blank" >RIV/60461373:22330/19:43918838 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Selection of Bacterial Strain for Lactic Acid Production from Straw Hydrolysate
Original language description
The objective of our project was to design and optimize the process of microbial production of lactic acid using an alternative, inexpensive substrate with achievement of lactic acid high yield. Fermentations were performed using a low-cost source of carbon, wheat straw hydrolysate. Identification tests of two strains of the genus Lactobacillus obtained from our laboratory were first performed to confirm or disprove their taxonomic classification. Firstly API 50 CHL test was performed. This test includes 50 biochemical tests, where fermentation of 49 saccharides in strips is assessed. For more accurate identification, MALDI-TOF was performed, which resulted in re-identification of the two strains as Lactobacillus casei 198 and Lactobacillus plantarum LHR14. Thereafter, MRS media screening tests were performed with these two strains, where, glucose, sucrose and xylose were tested as carbon sources. Further, inhibitors present in the straw hydrolysate such as coumaric acid, ferulic acid and syringaldehyde in concentrations ranging from 0.2 to 2.5 g.l-1 and potassium hydrogen phosphate at a concentration from 15 to 35 g.l-1 were added to the medium and growth and lactic acid production were tested.
Czech name
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Czech description
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Project
<a href="/en/project/LTACH17006" target="_blank" >LTACH17006: Production of biobutanol and succinic/lactic acid from lignocellulose biomass</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 7th International Conference on Chemical Technology
ISBN
978-80-88307-00-6
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
26-31
Publisher name
Czech Society of Industrial Chemistry
Place of publication
Prague
Event location
Mikulov
Event date
Apr 15, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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