SIGNALING PATHWAY FOR BUTANOL PRODUCTION IN CLOSTRIDIUM BEIJERINCKII NRRL B-598

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918863" target="_blank" >RIV/60461373:22330/19:43918863 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/19:PU133862

Result on the web

<a href="https://link.springer.com/article/10.1186/s12864-018-4805-8" target="_blank" >https://link.springer.com/article/10.1186/s12864-018-4805-8</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

SIGNALING PATHWAY FOR BUTANOL PRODUCTION IN CLOSTRIDIUM BEIJERINCKII NRRL B-598

Original language description

The unstable price of oil in the last decade is the reason for an increased interest in the sustainable production of biofuels. Microbial production is again becoming a competitive way of generating butanol, and it is a suitable alternative to the chemical production of oil. Still, there is plenty of room for the development of highly efficient phenotypes of various solvent-producing organisms. The key lies in the utilisation of systems biology and its models that can help us to fully understand the whole process on a molecular level in the selected organism, and to identify the genomic regions responsible for the desired phenotype features. Here, we present a dynamic model of butanol production in a promising solvent producer, Clostridium beijerinckii NRRL B-598. We utilised a wide range of data we collected over the last two years, including RNA-Seq gene expression, cell viability measured with flow cytometry, and metabolic activity measured with HPLC, to reconstruct a pathway involved in butanol production. Although solventogenic species are extensively studied organisms, we bring the first pathway of the strain C. beijerinckii NRRL B-598. We used an open platform, Cell Collective (cellcollective.org), where the model is publicly available under the name ‘Signalling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598’, version 1.1. To determine the properties of the pathway, we performed a series of static and dynamic analyses. The presented pathway is an ideal approach for exploring the possibilities of biofuel overproduction as it enables the activation or inactivation of individual genes and metabolites involved in the production of butanol. Not only can the acquired knowledge help with future engineering of the strain, but the results can be generalised for the microbial production of butanol in related species.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20901 - Industrial biotechnology

Project

<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 7th International Conference on Chemical Technology

ISBN

978-80-88307-00-6

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

46-52

Publisher name

Czech Society of Industrial Chemistry

Place of publication

Prague

Event location

Mikulov

Event date

Apr 15, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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