Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920333" target="_blank" >RIV/60461373:22330/19:43920333 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/19:00107959
Result on the web
<a href="https://www.mdpi.com/1420-3049/24/24/4540/htm" target="_blank" >https://www.mdpi.com/1420-3049/24/24/4540/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/molecules24244540" target="_blank" >10.3390/molecules24244540</a>

Result language
angličtina
Original language name
Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family
Original language description
The Photorhabdus species is a Gram-negative bacteria of the family Morganellaceae that is known for its mutualistic relationship with Heterorhabditis nematodes and pathogenicity toward insects. This study is focused on the characterization of the recombinant lectin PLL3 with an origin in P. laumondii subsp. laumondii. PLL3 belongs to the PLL family of lectins with a seven-bladed beta-propeller fold. The binding properties of PLL3 were tested by hemagglutination assay, glycan array, isothermal titration calorimetry, and surface plasmon resonance, and its structure was determined by X-ray crystallography. Obtained data revealed that PLL3 binds similar carbohydrates to those that the other PLL family members bind, with some differences in the binding properties. PLL3 exhibited the highest affinity toward L-fucose and its derivatives but was also able to interact with O-methylated glycans and other ligands. Unlike the other members of this family, PLL3 was discovered to be a monomer, which might correspond to a weaker avidity effect compared to homologous lectins. Based on the similarity to the related lectins and their proposed biological function, PLL3 might accompany them during the interaction of P. laumondii with both the nematode partner and the insect host.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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