In vitro quantification of the effects of IP6 and other small polyanions on immature hiv-1 particle assembly and core stability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43920757" target="_blank" >RIV/60461373:22330/20:43920757 - isvavai.cz</a>
Result on the web
<a href="https://jvi.asm.org/content/94/20/e00991-20" target="_blank" >https://jvi.asm.org/content/94/20/e00991-20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JVI.00991-20" target="_blank" >10.1128/JVI.00991-20</a>
Alternative languages
Result language
angličtina
Original language name
In vitro quantification of the effects of IP6 and other small polyanions on immature hiv-1 particle assembly and core stability
Original language description
Proper assembly and disassembly of both immature and mature HIV-1 hexameric lattices are critical for successful viral replication. These processes are facilitated by several host-cell factors, one of which is myo-inositol hexaphosphate (IP6). IP6 participates in the proper assembly of Gag into immature hexameric lattices and is incorporated into HIV-1 particles. Following maturation, IP6 is also likely to participate in stabilizing capsid protein-mediated mature hexameric lattices. Although a structural-functional analysis of the importance of IP6 in the HIV-1 life cycle has been reported, the effect of IP6 has not yet been quantified. Using two in vitro methods, we quantified the effect of IP6 on the assembly of immature-like HIV-1 particles, as well as its stabilizing effect during disassembly of mature-like particles connected with uncoating. We analyzed a broad range of molar ratios of protein hexamers to IP6 molecules during assembly and disassembly. The specificity of the IP6-facilitated effect on HIV-1 particle assembly and stability was verified by K290A, K359A, and R18A mutants. In addition to IP6, we also tested other polyanions as potential assembly cofactors or stabilizers of viral particles. © 2020 American Society for Microbiology.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/GA20-19906S" target="_blank" >GA20-19906S: Modulation of capsid protein hexameric lattice stability: a promising target for HIV-1 inhibition</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Virology
ISSN
0022-538X
e-ISSN
—
Volume of the periodical
94
Issue of the periodical within the volume
20
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
—
UT code for WoS article
000579827700010
EID of the result in the Scopus database
2-s2.0-85092332116