Comparison of lactic acid production by L. casei in batch, fed-batch and continuous cultivation, testing the use of feather hydrolysate as a complex nitrogen source
Result description
A comprehensive comparison of the main fermentation parameters, productivity, yield and final Llactic acid concentration, obtained through batch, fed-batch and continuous cultivations using Lactobacillus casei CCDM 198 and a model cultivation medium was carried out. Using this data, a pulse-feed fed-batch process was established for testing chicken feather hydrolysate as a replacement for all complex nitrogen sources (yeast and beef extracts and peptone) in the medium. As comparably high values of productivity (about 4.0 g/L/h) and yield (about 98 %) were reached under all cultivation conditions, the maximum final Llactic acid concentration (116.5 g/L), as achieved through pulse-feed fed-batch fermentation, was chosen as the most important criterion for process selection. Fed-batch cultivation with chicken feather hydrolysate as both a complex nitrogen source and a neutralizing agent for maintaining constant culture pH yielded half the concentration of L-lactic acid compared to the model medium. We demonstrate here that chicken feather hydrolysate has potential for use in the production of L-lactic acid but its utilization requires further optimization. © 2020 by the authors.
Keywords
Process designLactobacillus caseiLactic acidHomofermentative fermentationChicken feather hydrolysate
The result's identifiers
Result code in IS VaVaI
Result on the web
http://www.scielo.br/pdf/babt/v63/1516-8913-babt-63-e20190151.pdf
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Comparison of lactic acid production by L. casei in batch, fed-batch and continuous cultivation, testing the use of feather hydrolysate as a complex nitrogen source
Original language description
A comprehensive comparison of the main fermentation parameters, productivity, yield and final Llactic acid concentration, obtained through batch, fed-batch and continuous cultivations using Lactobacillus casei CCDM 198 and a model cultivation medium was carried out. Using this data, a pulse-feed fed-batch process was established for testing chicken feather hydrolysate as a replacement for all complex nitrogen sources (yeast and beef extracts and peptone) in the medium. As comparably high values of productivity (about 4.0 g/L/h) and yield (about 98 %) were reached under all cultivation conditions, the maximum final Llactic acid concentration (116.5 g/L), as achieved through pulse-feed fed-batch fermentation, was chosen as the most important criterion for process selection. Fed-batch cultivation with chicken feather hydrolysate as both a complex nitrogen source and a neutralizing agent for maintaining constant culture pH yielded half the concentration of L-lactic acid compared to the model medium. We demonstrate here that chicken feather hydrolysate has potential for use in the production of L-lactic acid but its utilization requires further optimization. © 2020 by the authors.
Czech name
—
Czech description
—
Classification
Type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Result continuities
Project
LTACH17006: Production of biobutanol and succinic/lactic acid from lignocellulose biomass
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Brazilian Archives of Biology and Technology
ISSN
1516-8913
e-ISSN
—
Volume of the periodical
63
Issue of the periodical within the volume
May 2020
Country of publishing house
BR - BRAZIL
Number of pages
12
Pages from-to
—
UT code for WoS article
000535198100001
EID of the result in the Scopus database
2-s2.0-85086379844
Basic information
Result type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
OECD FORD
Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Year of implementation
2020