Authentication of Panax Ginseng Based Nutraceuticals using ´Chemical Markers´ Strategy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43922843" target="_blank" >RIV/60461373:22330/21:43922843 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Authentication of Panax Ginseng Based Nutraceuticals using ´Chemical Markers´ Strategy

Original language description

This study is focused on the development of a method for authentication of nutraceuticals containing high value ginseng, Panax ginseng. For this purpose, analytical strategy based on metabolomic fingerprinting using ultra high performance liquid chromatography – high resolution mass spectrometry (UHPLC-HRMS) was applied. Worth to notice, that several representatives of Panax genus exist differing in content in biologically active substances such as ginsenosides, gypenosides, majorosides etc.; moreover, there are other plants which contain them, thus they can be used as undeclared substitutes. Application of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) with data processing using advanced statistical methods (principal component analysis and partial least square discriminant analysis) enabled to find 105 specific markers for Panax ginseng, 65 for Panax notoginseng, 120 for Withania somnifera, 99 for Eleutherococcus senticosus and 109 for Gynostemma pentaphyllum in aqueous methanolic (MeOH:water, 80:20) extracts of these herbs. In the next step, extracts of the most common ingredients (green tea, ginger, peppermint, rooibos, etc.) contained in herbal teas were analyzed and the specificity of these markers was verified. It was found that only 5 markers of Panax ginseng (ESI+: m/z 974.5323 (retention time 4.57 min), m/z 1109.6108 (RT 4.62 min); ESI-: m/z 793.435 (RT 5.11 min), m/z 955.4903 (RT 4.59 min), m/z 1077.585 (RT 4.67 min)), 2 markers of Panax notoginseng, 30 markers of Withania somnifera, 6 markers of Eleutherococcus senticosus and 20 markers of Gynostemma pentaphyllum are specific for them. The analysis of these specific markers allowed authentication of food supplements containing Panax ginseng, especially herbal teas with Panax ginseng content. The presence of Panax ginseng was confirmed in 7 of 15 analyzed food supplements with its declared content. Characteristic markers of Panax notoginseng, Withania somnifera, Eleutherococcus senticosus and Gynostemma pentaphyllum were not found in these food supplements.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů