Evaluation of the antimicrobial efficacy of n-acetyl-l-cysteine, rhamnolipids, and usnic acid—novel approaches to fight food-borne pathogens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923685" target="_blank" >RIV/60461373:22330/21:43923685 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1422-0067/22/21/11307/html" target="_blank" >https://www.mdpi.com/1422-0067/22/21/11307/html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms222111307" target="_blank" >10.3390/ijms222111307</a>

Result language

angličtina

Original language name

Evaluation of the antimicrobial efficacy of n-acetyl-l-cysteine, rhamnolipids, and usnic acid—novel approaches to fight food-borne pathogens

Original language description

In the food industry, the increasing antimicrobial resistance of food-borne pathogens to conventional sanitizers poses the risk of food contamination and a decrease in product quality and safety. Therefore, we explored alternative antimicrobials N-acetyl-L-cysteine (NAC), rhamnolipids (RLs), and usnic acid (UA) as a novel approach to prevent biofilm formation and reduce existing biofilms formed by important food-borne pathogens (three strains of Salmonella enterica and two strains of Escherichia coli, Listeria monocytogenes, Staphylococcus aureus). Their effectiveness was evaluated by determining minimum inhibitory concentrations needed for inhibition of bacterial growth, biofilm formation, metabolic activity, and biofilm reduction. Transmission electron microscopy and confocal scanning laser microscopy followed by image analysis were used to visualize and quantify the impact of tested substances on both planktonic and biofilm-associated cells. The in vitro cytotoxicity of the substances was determined as a half-maximal inhibitory concentration in five different cell lines. The results indicate relatively low cytotoxic effects of NAC in comparison to RLs and UA. In addition, NAC inhibited bacterial growth for all strains, while RLs showed overall lower inhibition and UA inhibited only the growth of Gram-positive bacteria. Even though tested substances did not remove the biofilms, NAC represents a promising tool in biofilm prevention. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-15936S" target="_blank" >GA17-15936S: Interaction of nanoparticles modified by natural compounds with biofilms of pathogenic microorganisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

—

Volume of the periodical

22

Issue of the periodical within the volume

21

Country of publishing house

CH - SWITZERLAND

Number of pages

21

Pages from-to

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UT code for WoS article

000720473300001

EID of the result in the Scopus database

2-s2.0-85117319359