The influence of colouring materials and artificial ageing on the occurrence of nonspecific peptides in protein binders

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43924508" target="_blank" >RIV/60461373:22330/22:43924508 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11410/22:10449849

Result on the web

<a href="https://doi.org/10.1016/j.microc.2022.107258" target="_blank" >https://doi.org/10.1016/j.microc.2022.107258</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2022.107258" target="_blank" >10.1016/j.microc.2022.107258</a>

Result language

angličtina

Original language name

The influence of colouring materials and artificial ageing on the occurrence of nonspecific peptides in protein binders

Original language description

The aim of this work was to identify the influence of selected pigments and artificial ageing on the occurrence of nonspecific peptides in protein binders used in artworks. For this purpose, two groups of pigments – inorganic (cadmium yellow, chalk, and ochre) and organic (indigo, carmine, and madder lake) were selected. The model samples containing protein binders (casein binder, gelatine, yolk and whole egg binder) mixed with the indicated pigments in final concentration of 1 % were exposed to the conditions causing accelerated ageing (UV light, higher temperature and relative humidity). The samples were analysed using two mass spectrometry techniques – LC-ESI-Q-TOF MS (Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight Mass Spectrometry) and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry). The results from LC-ESI-Q-TOF MS were searched for sites where nonspecific cleavage of proteins occurred and for types and the number of modifications. MALDI-TOF MS data were subjected to the Principal Component Analysis (PCA). Beside amino acid modifications and spatial conformations another way of protein binder deterioration was described in this paper. It was found that pigments can influence the number of nonspecific peptide fragments and may thus led to binder degradation, or have a stabilizing effect. The data obtained from artificial ageing show that ageing may also affect the occurrence of nonspecific peptides and, that this effect strongly depends on the presence of the specific pigment or dye.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Volume of the periodical

177

Issue of the periodical within the volume

Červenec 2022

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

107258

UT code for WoS article

000875675900001

EID of the result in the Scopus database

2-s2.0-85124224238