Membrane proteins and proteomics of Cronobacter sakazakii cells: Reliable method for identification and subcellular localization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925298" target="_blank" >RIV/60461373:22330/22:43925298 - isvavai.cz</a>
Result on the web
<a href="https://journals.asm.org/doi/10.1128/aem.02508-21" target="_blank" >https://journals.asm.org/doi/10.1128/aem.02508-21</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/aem.02508-21" target="_blank" >10.1128/aem.02508-21</a>
Alternative languages
Result language
angličtina
Original language name
Membrane proteins and proteomics of Cronobacter sakazakii cells: Reliable method for identification and subcellular localization
Original language description
This study is the first to fractionate Cronobacter sakazakii cells into outer membrane, inner membrane, periplasmic, and cytosolic fractions as the basis for improved proteome mapping. A novel method was designed to prepare the fractionated samples for protein identification. The identification was performed via one-dimensional electrophoresis-liquid chromatography electrospray ionization tandem mass spectrometry. To determine the subcellular localization of the identified proteins, we developed a novel Python-based script (Subcelloc) that combines three web-based tools, PSORTb 3.0.2, CELLO 2.5, and UniProtKB. Applying this approach enabled us to identify 1,243 C. sakazakii proteins, which constitutes 28 % of all predicted proteins and 49 % of all theoretically expressed outer membrane proteins. These results represent a significant improvement on previous attempts to map the C. sakazakii proteome and could provide a major step forward in the identification of Cronobacter virulence factors.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied and Environmental Microbiology
ISSN
0099-2240
e-ISSN
1098-5336
Volume of the periodical
88
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
1-17
UT code for WoS article
000784536500002
EID of the result in the Scopus database
2-s2.0-85129971235