Analytical strategy for identification of unknown transformation products of mycotoxins after their decontamination by pulsed electric field technology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925448" target="_blank" >RIV/60461373:22330/22:43925448 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Analytical strategy for identification of unknown transformation products of mycotoxins after their decontamination by pulsed electric field technology

Original language description

Mycotoxin contamination of cereals is a problem of a global scope. Different decontamination technologies are in use today, including the pulsed electric field (PEF). Our recent research on this topic revealed significant influence of PEF on decontamination of mycotoxins present in cereals. The overall reduction of trichothecenes, zearalenone, enniatins, beauvericin, and tentoxin in barley was up to 31, 48, 84, 36 and 46%, respectively. As we found out, besides the increased extractability into the electrolyte (water), the major reason of this reduction was mycotoxins’ degradation and/or transformation into the different reaction products. As the identification and characterization of these unknown reaction products is of a major importance in terms of follow up toxicity and risk assessment, we developed an analytical strategy enabling characterization and confirmation of these products by using the tools of advanced analytical chemistry, including the in silico modelling and high resolution mass spectrometry (HRMS). For creating the database of predicted reaction products, Zeneth software was used, and these compounds were further pre-screened by ultra-high performance liquid chromatography coupled with Q-orbitrap HRMS. Positive hints of this prescreening were further verified by evaluation of their retention times, and compliance of their HRMS/MS spectra with the in silico predictions. The determined degradation / transformation products of mycotoxins were mostly products of hydrolysis, elimination and/or oxidation.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LM2018100" target="_blank" >LM2018100: Infrastructure for Promoting Metrology in Food and Nutrition in the Czech Republic</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů