Myristoyl switch at the plasma membrane serves as the trigger for Gag cleavage and oligomerization of the matrix protein in the Mason-Pfizer monkey virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43932689" target="_blank" >RIV/60461373:22330/23:43932689 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22340/23:43932689 RIV/60461373:22810/23:43932689

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Myristoyl switch at the plasma membrane serves as the trigger for Gag cleavage and oligomerization of the matrix protein in the Mason-Pfizer monkey virus

Original language description

Despite low sequence homology, retroviral structural proteins exhibit remarkable similarity in their secondary structures and interactions. These shared characteristics extend to their assembly processes, albeit with intriguing variations. While most retroviruses, including the well-known HIV-1, utilize the plasma membrane (PM) as a scaffold promoting the assembly of immature particles, which occurs simultaneously with budding and initiation of maturation, certain retroviruses, like the Mason-Pfizer monkey virus (M-PMV), undertake assembly within the cytoplasm. Consequently, M-PMV particles must be transported to the PM in their immature state, necessitating careful orchestration to prevent premature protease activation and specific cleavage events until they reach the PM. This crucial interaction is triggered by a bipartite signal composed of a cluster of basic residues in the matrix (MA) domain of the Gag polyprotein and a myristoyl moiety attached to MA at its N-terminus. In this study, we present evidence that myristoyl exposure from the MA core and its insertion into the PM bilayer occurs in M-PMV. Employing a combination of experimental techniques, we demonstrate that this process induces a structural change at the C-terminus of MA, supporting the efficient cleavage of MA from the downstream region of M-PMV Gag. These findings suggest that, in addition to the known effect of the myristoyl switch of on Gag multimerization and particle assembly in HIV-1, the myristoyl switch may also play a regulatory role in initiating sequential Gag cleavage events within immature M-PMV particles.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů