Calmodulin involvement in the late phase life cycle of Mason-Pfizer monkey virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932633" target="_blank" >RIV/60461373:22330/25:43932633 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22810/25:43932633

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Calmodulin involvement in the late phase life cycle of Mason-Pfizer monkey virus

Original language description

Some viruses exploit host calcium signaling pathways to regulate key steps in their life cycle. Calmodulin (CaM), a ubiquitous calcium-sensing protein, is known to interact with several viral proteins, modulating processes such as viral replication and assembly. Although the interaction between CaM and HIV-1 matrix (MA) protein has been characterized in detail, the exact role of CaM in the replication of retroviruses remains unclear.In this study, we investigated the interaction between the MA protein of Mason-Pfizer monkey virus (M-PMV) and CaM. Using microscale thermophoresis (MST), we demonstrated that M-PMV MA binds CaM in a calcium-dependent manner. Co-immunoprecipitation assays from HEK293T cells co-expressing the M-PMV structural polyprotein Gag and HA-tagged CaM confirmed the relevance of this interaction in vivo. Furthermore, we found that CaM enhances proteolytic cleavage of myristoylated M-PMV MAPP, an MA construct extended with a downstream region of the phosphoprotein that mimics the M-PMV Gag precursor. This suggests that CaM binding facilitates the myristoyl switch of M-PMV MA. This is supported by liposome binding experiments showing that CaM promotes MA association with liposomes mimicking the cytoplasmic membrane. Formation of CaM–MA complexes was further confirmed by protein crosslinking followed by mass spectrometric analysis. Together, our findings provide new insights into the interaction between CaM and M-PMV MA, highlighting a potential regulatory mechanism during the late phase of the retroviral life cycle.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů