Metody pro charakterizaci konjugátů radionuklid - ligand - protilátka

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F05%3A00014550" target="_blank" >RIV/60461373:22340/05:00014550 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Metody pro charakterizaci konjugátů radionuklid - ligand - protilátka

Original language description

At present, the importance of complexes, when the radionuclide was transported to tumor via antibody labeled by a and b emitters, increasingly grows in the targeted therapy of cancers. We were dealing with the study of the human immunoglobulin G (IgG) and anti-CD 20 conjugations with the bicyclic anhydride of the diethylentriamino-N,N,N,N,N-pentaacetate acid (cDTPAA) and labeling of prepared conjugates by 90Y (carrier-free) and 177Lu radionuclides. The HPLC - SEC method, the ultrafiltation and the gel was used for separationof the antibody - DTPA conjugate and free DTPA. The characterization of the bifunctional chelaton bond to antibody was carried out by FTIR spectroscopy.

Czech name

Metody pro charakterizaci konjugátů radionuklid - ligand - protilátka

Czech description

At present, the importance of complexes, when the radionuclide was transported to tumor via antibody labeled by a and b emitters, increasingly grows in the targeted therapy of cancers. We were dealing with the study of the human immunoglobulin G (IgG) and anti-CD 20 conjugations with the bicyclic anhydride of the diethylentriamino-N,N,N,N,N-pentaacetate acid (cDTPAA) and labeling of prepared conjugates by 90Y (carrier-free) and 177Lu radionuclides. The HPLC - SEC method, the ultrafiltation and the gel was used for separationof the antibody - DTPA conjugate and free DTPA. The characterization of the bifunctional chelaton bond to antibody was carried out by FTIR spectroscopy.

Type

O - Miscellaneous

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA104%2F03%2F0499" target="_blank" >GA104/03/0499: Technology of the preparations of radionuclides and their labelled compounds for applications in medicine and pharmacy with the use of reactor LVR-15</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů