Time-Dependent Stokes Shifts of Fluorescent Dyes in the Hydrophobic Backbone Region of a Phospholipid Bilayer: Combination of Fluorescence Spectroscopy and Ab Initio Calculations
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F07%3A00018427" target="_blank" >RIV/60461373:22340/07:00018427 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/07:00084819 RIV/61388963:_____/07:00084819
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Time-Dependent Stokes Shifts of Fluorescent Dyes in the Hydrophobic Backbone Region of a Phospholipid Bilayer: Combination of Fluorescence Spectroscopy and Ab Initio Calculations
Original language description
We explored the time-dependent Stokes shifts of fluorescent dyes containing an anthroyloxy chromophore (2-AS, 9-AS, and 16-AP) in bilayers composed of palmitoyl-oleoyl-phosphatidylcholine. The obtained data revealed a nontrivial solvation response of these dyes, which are located in the backbone region of the bilayer with a gradually increasing depth. For comparison, steady-state emission spectra in the neat solvents of various polarities and viscosities were also recorded. The results indicate that onthe short picosecond time scale the AS dyes undergo complex photophysics including formation of states with a charge-transfer character. This observation is supported by ab initio calculations of the excited states of 9-methylanthroate. The slower nanosecond part of the relaxation process can be attributed to the solvation response of the dyes. A slowdown in solvent relaxation is observed upon moving toward the center of the bilayer. A mechanism similar to preferential solvation present
Czech name
Časově závislý Stokesův posun fluorescenčních značek v hydrofobní části fosfolipidových dvojvrstev: kombinace fluorescenční spektroskopie a ab initio výpočtů
Czech description
Byla stuována fotochemie fluorescenčních značek typu n-AS a n-AP pomocí ab initio metod. Zároveň byly proměřeny fluorescenční charakteristiky těchto značek v různých orientovaných prostředích.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF PHYSICAL CHEMISTRY B
ISSN
1520-6106
e-ISSN
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Volume of the periodical
111
Issue of the periodical within the volume
21
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
12477-12486
UT code for WoS article
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EID of the result in the Scopus database
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