On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F09%3A00022071" target="_blank" >RIV/60461373:22340/09:00022071 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

Original language description

Radio-frequency impedance based biomass measurements represent a relatively accurate and reliable method for on-line quantification of viable cellular biomass. For the living biomass concentration determination, a linear correlation between biomass concentration and capacitance is generally assumed. However, this assumption is not always valid as e.g. the presence of intracellular lipid inclusions may violate the assumption of linear correlation. Therefore, the main objective of this study was to examine the potential of the sensor for on-line in-situ monitoring of intracellular biopolymer content (PHA) in bacterial cells. It was experimentally confirmed that the loss of linearity between dry cell weight and capacitance takes place also in the case ofthe Pseudomonas putida culture. The resulting nonlinear behaviour can be used for the design of a novel biopolymer content estimator based on the capacitance and offgas composition measurements.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

New Biotechnology

ISSN

1871-6784

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

S1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

2

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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