Vibrational circular dichroism study of solvent- and temperature-induced conformational changes in poly-gamma-benzyl-L-glutamate and poly-beta-benzyl-L-aspartate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F13%3A43895079" target="_blank" >RIV/60461373:22340/13:43895079 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.vibspec.2013.01.007" target="_blank" >http://dx.doi.org/10.1016/j.vibspec.2013.01.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vibspec.2013.01.007" target="_blank" >10.1016/j.vibspec.2013.01.007</a>

Result language

angličtina

Original language name

Vibrational circular dichroism study of solvent- and temperature-induced conformational changes in poly-gamma-benzyl-L-glutamate and poly-beta-benzyl-L-aspartate

Original language description

Vibrational circular dichroism (VCD) spectroscopy was used to study the effect of the different composition of mixed solvents and temperature on the conformation and aggregation states of two synthetically prepared polypeptides, poly-gamma-benzyl-L-glutamate (PBLG) and poly-beta-benzyl-L-aspartate (PBLA). Additions of trifluoroacetic acid (TFA) into a solution of heligenic solvents trichloromethane and benzene-d6 caused the conformational change from the alpha-helical to polyproline II-like for both ofthe polypeptides, which represented interesting transition previously mostly observed in aqueous solutions rather than in organic solvents. The VCD method proved a lower stability of the alpha-helical conformation of PBLA than PBLG and the structural differences between these polypeptides. The variation of temperature in the region 13-50 degrees C induced atypical conformational transformations in the PBLG/trichloromethane/TFA and PBLG/benzene-d6/TFA systems. The usually more stable alph

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP208%2F11%2F0105" target="_blank" >GAP208/11/0105: Expanding the Optical Activity Method to the Realm of Biomolecules</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Vibrational Spectroscopy

ISSN

0924-2031

e-ISSN

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Volume of the periodical

66

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

1-7

UT code for WoS article

000319097100001

EID of the result in the Scopus database

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