Controlling the time evolution of mAb N-linked glycosylation, Part I: Microbioreactor experiments
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43902868" target="_blank" >RIV/60461373:22340/16:43902868 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/btpr.2305" target="_blank" >http://dx.doi.org/10.1002/btpr.2305</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/btpr.2305" target="_blank" >10.1002/btpr.2305</a>
Alternative languages
Result language
angličtina
Original language name
Controlling the time evolution of mAb N-linked glycosylation, Part I: Microbioreactor experiments
Original language description
Vyplňuje se automaticky N-linked glycosylation is of key importance for the efficacy of many biotherapeutic proteins such as monoclonal antibodies (mAbs). Media components and cell culture conditions have been shown to significantly affect N-linked glycosylation during the production of glycoproteins using mammalian cell fed-batch cultures. These parameters inevitably change in modern industrial processes with concentrated feed additions and cell densities beyond 2 x 10(7) cells/mL. In order to control the time-dependent changes of protein glycosylation, an automated microbioreactor system was used to investigate the effects of culture pH, ammonia, galactose, and manganese chloride supplementation on nucleotide sugars as well as mAb N-linked glycosylation in a time-dependent way. Two different strategies comprising of a single shift of culture conditions as well as multiple media supplementations along the culture duration were applied to obtain changing and constant glycosylation profiles. The different feeding approaches enabled constant glycosylation patterns throughout the entire culture duration at different levels. By modulating the time evolution of the mAb glycan pattern, not only the endpoint but also the ratios between different glycosylation structures could be modified.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CI - Industrial chemistry and chemical engineering
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biotechnology Progress
ISSN
1520-6033
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1123-1134
UT code for WoS article
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EID of the result in the Scopus database
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