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Discrete Turing Patterns in Coupled Reaction Cells in a Cyclic Array

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43903030" target="_blank" >RIV/60461373:22340/16:43903030 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s11144-016-1004-y" target="_blank" >http://dx.doi.org/10.1007/s11144-016-1004-y</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11144-016-1004-y" target="_blank" >10.1007/s11144-016-1004-y</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Discrete Turing Patterns in Coupled Reaction Cells in a Cyclic Array

  • Original language description

    Our work is focused on experimental observation of nonuniform stationary states (discrete Turing patterns) in a cyclic coupled cell array with glycolytic oscillatory reaction. Mutual mass exchange between two stirred reaction cells is realized by using a peristaltic pump delivering reciprocally the reaction mixture in both directions. The cells are fed by solutions of yeast extract and D-glucose. The cyclic system is formed by the two cells and the tubing, connecting the cells, acting as additional two reactors because of a non-negligible transport time. Experiments are conducted under 45oC and 50oC to thermally activate positive and negative feedback loops of the enzyme phosphofructokinase and thus achieve conditions appropriate for occurrence of the patterns. Dynamical state in one cell of the ring is measured using UV-Vis spectrophotometer reading absorbance levels of adenosine pool together with NAD+ (260nm) and absorbance level of NADH (340 nm). We report transitions between uniform stationary states, nonuniform stationary states (discrete Turing patterns) and uniform oscillations. We also performed analysis of stability and bifurcations of stationary states in a model of four coupled cells in a cyclic array using a core kinetic model of glycolysis. By examining the space of relevant parameters, we found conditions for occurrence of discrete Turing patterns consistent with our experiments. Solution diagrams are used to display the stationary concentration of ADP as a function of the inhibition rate coefficient and to explain the mechanism by which the discrete Turing patterns occur.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CI - Industrial chemistry and chemical engineering

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA15-17367S" target="_blank" >GA15-17367S: Dynamics of complex reaction networks in enzyme reactors and photobioreactors</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Reaction Kinetics Mechanisms and Catalysis

  • ISSN

    1878-5190

  • e-ISSN

  • Volume of the periodical

    118

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    16

  • Pages from-to

    99-114

  • UT code for WoS article

    000376107700010

  • EID of the result in the Scopus database