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Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43916534" target="_blank" >RIV/60461373:22340/18:43916534 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.bej.2017.12.004" target="_blank" >10.1016/j.bej.2017.12.004</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

  • Original language description

    Transfer of mammalian cell culture processes across stirred and aerated bioreactor scales is a delicate task, frequently leading to different conditions across scales. To provide a more rational reasoning behind scale-up of mammalian cell cultures, physical characterization of bioreactors with working volumes of 15 mL, 3 L, 270 L, 5′000 L and 15′000 L was carried out using a combination of computational and experimental methods. Maximum hydrodynamic stress, mixing time and oxygen mass transfer coefficients were experimentally determined for all bioreactor scales. Computational fluid dynamic (CFD) simulations based on Reynolds-averaged Navier‐Stokes equation coupled with bubble size population balance equations were used to determine local as well as average hydrodynamic stresses and mass transfer coefficients. Furthermore, mixing times were determined by simulated tracer experiments. All calculations are well in agreement with experimentally measured values, thereby providing a validation of the CFD simulations. This integrated experimental and modeling methodology represents a valuable tool for a Quality-by-Design approach enabling the transfer of mammalian cell cultures in-between reactor scales, even for geometrically different reactors. Additionally, this study provides a rational framework to transfer an operating space developed at small scale to larger scales. © 2017 Elsevier B.V.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20401 - Chemical engineering (plants, products)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biochemical Engineering Journal

  • ISSN

    1369-703X

  • e-ISSN

  • Volume of the periodical

    131

  • Issue of the periodical within the volume

    Neuveden

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    84-94

  • UT code for WoS article

    000426028600011

  • EID of the result in the Scopus database

    2-s2.0-85041128222