Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43916534" target="_blank" >RIV/60461373:22340/18:43916534 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bej.2017.12.004" target="_blank" >10.1016/j.bej.2017.12.004</a>
Alternative languages
Result language
angličtina
Original language name
Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale
Original language description
Transfer of mammalian cell culture processes across stirred and aerated bioreactor scales is a delicate task, frequently leading to different conditions across scales. To provide a more rational reasoning behind scale-up of mammalian cell cultures, physical characterization of bioreactors with working volumes of 15 mL, 3 L, 270 L, 5′000 L and 15′000 L was carried out using a combination of computational and experimental methods. Maximum hydrodynamic stress, mixing time and oxygen mass transfer coefficients were experimentally determined for all bioreactor scales. Computational fluid dynamic (CFD) simulations based on Reynolds-averaged Navier‐Stokes equation coupled with bubble size population balance equations were used to determine local as well as average hydrodynamic stresses and mass transfer coefficients. Furthermore, mixing times were determined by simulated tracer experiments. All calculations are well in agreement with experimentally measured values, thereby providing a validation of the CFD simulations. This integrated experimental and modeling methodology represents a valuable tool for a Quality-by-Design approach enabling the transfer of mammalian cell cultures in-between reactor scales, even for geometrically different reactors. Additionally, this study provides a rational framework to transfer an operating space developed at small scale to larger scales. © 2017 Elsevier B.V.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20401 - Chemical engineering (plants, products)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical Engineering Journal
ISSN
1369-703X
e-ISSN
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Volume of the periodical
131
Issue of the periodical within the volume
Neuveden
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
84-94
UT code for WoS article
000426028600011
EID of the result in the Scopus database
2-s2.0-85041128222