Digital Image Analysis for Morphological State Characterization of a Culture of Filamentous Microorganisms in Production of Antibiotics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F19%3A43919043" target="_blank" >RIV/60461373:22340/19:43919043 - isvavai.cz</a>
Result on the web
<a href="https://www.scientific.net/JBBBE.43.74" target="_blank" >https://www.scientific.net/JBBBE.43.74</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4028/www.scientific.net/JBBBE.43.74" target="_blank" >10.4028/www.scientific.net/JBBBE.43.74</a>
Alternative languages
Result language
angličtina
Original language name
Digital Image Analysis for Morphological State Characterization of a Culture of Filamentous Microorganisms in Production of Antibiotics
Original language description
The paper deals with the application of specific methods of digital image analysis for the monitoring of morphological changes in cultures of filamentous microorganisms. First, a sequence of digital image preprocessing and processing steps is proposed for the treatment of microscopic images of a filamentous culture. The preprocessing step include band pass filtering by the Difference of Gaussians filter featuring a novel approach to the task of parameters tuning, as well as the optimization of image porosity and image objects separation quality. In the processing step, the resulting enhanced images are subject to morphological state characterization using a set of several standard and modified morphological parameters. Descriptions of morphological states of different complexity are then discussed varying from the standard set of mean values of parameters to the set of parameters in their full histogram of value frequencies (distribution) form. For such complex descriptions also new fashions of graphical representation of results without loss of information are compared. The potential of the proposed full description of morphological behavior of the culture is demonstrated using a set of microscopic images taken during an industrial antibiotics production cultivation using a microorganism belonging into the Streptomyces genus. Finally, the cluster analysis is proposed for further automatic quantitative classification and interpretation of complex description of metabolic states.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20205 - Automation and control systems
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biomimetics, Biomaterials and Biomedical Engineering
ISSN
2296-9845
e-ISSN
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Volume of the periodical
43
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
74-83
UT code for WoS article
000497982400007
EID of the result in the Scopus database
2-s2.0-85076966935