Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F20%3A43920293" target="_blank" >RIV/60461373:22340/20:43920293 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/20:00534343 RIV/68378041:_____/20:00534343 RIV/00216208:11110/20:10417008 RIV/00216275:25310/20:39916563
Result on the web
<a href="https://www.mdpi.com/2297-8739/7/3/49/htm" target="_blank" >https://www.mdpi.com/2297-8739/7/3/49/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/separations7030049" target="_blank" >10.3390/separations7030049</a>

Result language
angličtina
Original language name
Analysis of chondroitin/dermatan sulphate disaccharides using high-performance liquid chromatography
Original language description
Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 μg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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