Advanced microextraction techniques for the analysis of amphetamines in human breast milk and their comparison with conventional methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43922505" target="_blank" >RIV/60461373:22340/22:43922505 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22810/22:43922505 RIV/00023752:_____/22:43920778 RIV/00216208:11160/22:10452115

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0731708521006609?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0731708521006609?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2021.114549" target="_blank" >10.1016/j.jpba.2021.114549</a>

Result language

angličtina

Original language name

Advanced microextraction techniques for the analysis of amphetamines in human breast milk and their comparison with conventional methods

Original language description

Breast milk analysis provides useful information about acute newborn exposure to harmful substances, such as psychoactive drugs abused by a nursing mother. Since breast milk represents a complex matrix with large amounts of interfering compounds, a comprehensive sample pre-treatment is necessary. This work focuses on determination of amphetamines and synthetic cathinones in human breast milk by microextraction techniques (liquid-phase microextraction and electromembrane extraction), and their comparison to more conventional treatment methods (protein precipitation, liquid-liquid extraction, and saltingout assisted liquid-liquid extraction). The aim of this work was to optimize and validate all the extraction procedures and thoroughly assess their advantages and disadvantages with special regard to their routine clinical use. The applicability of the extractions was further verified by the analysis of six real samples collected from breastfeeding mothers suspected of amphetamine abuse. The membrane microextraction techniques turned out to be the most advantageous as they required low amounts of organic solvents but still provided efficient sample clean-up, excellent quantification limit (0.5 ng mL(-1)), and good recovery (81-91% and 40-89% for electromembrane extraction and liquid-phase microextraction, respectively). The traditional liquid-liquid extraction as well as the salting-out assisted liquid-liquid extraction showed comparable recoveries (41-85% and 63-88%, respectively), but higher quantification limits (2.5 ng mL(-1) and 5 ng mL(-1), respectively). Moreover, these methods required multiple operating steps and were time consuming. Protein precipitation was fast and simple, but it demonstrated poor sample clean-up, low recovery (56-58%) and high quantification limit (5 ng mL(-1)). Based on the overall results, microextraction methods can be considered promising candidates, even for routine laboratory use. (C) 2021 Elsevier B.V. All rights reserved.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

1873-264X

Volume of the periodical

210

Issue of the periodical within the volume

FEB 20 2022

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

114549

UT code for WoS article

000741479200003

EID of the result in the Scopus database

2-s2.0-85122263042