Effect of protease XXIII on selenium species interconversion during their extraction from biological samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43924394" target="_blank" >RIV/60461373:22340/22:43924394 - isvavai.cz</a>
Alternative codes found
RIV/26722861:_____/22:N0000004
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0889157521004609?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0889157521004609?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2021.104260" target="_blank" >10.1016/j.jfca.2021.104260</a>
Alternative languages
Result language
angličtina
Original language name
Effect of protease XXIII on selenium species interconversion during their extraction from biological samples
Original language description
Protease XIV is the most common used agent for selenium species extraction from biological samples. In this work, the interconversion of selenocystine during extraction with Tris−HCl buffer containing protease XIV was demonstrated. The 29 % of selenocystine was converted to species with retention time corresponding to Se-methyl-selenocysteine. In addition, selenomethionine, selenoethionine, and Se-methyl-selenocysteine were oxidized to their oxo-analogues when 0.1 % Triton X-100 or 0.1 % sodium dodecyl sulphate was used for improvement of extraction efficiency. The interconversion of these species was suppressed by using a protease XXIII, which application for the extraction of selenium species is described here for the first time. The method for speciation analysis of nine Se species (selenate, selenite, selenomethionine, selenocystine, Se-methyl-selenocysteine, selenoethionine, trimethylselenonium, methylselenocysteine oxide, and selenomethionine Se-oxide) using extraction with protease XXIII was developed and validated (limits of quantitation 0.21–0.70 μg/L Se, linearity up to 1000 μg/L Se, spike recovery 92.3–110.1 %). It has also been shown that the extraction efficiency of protease XXIII (average 53 %) is only slightly lower in comparison to protease XIV (average 65 %). The main Se species in biological samples, selenomethionine, selenocystine, and Se-methyl-selenocysteine, were stable in extract for three days at room temperature. © 2021 Elsevier Inc.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LTC20014" target="_blank" >LTC20014: Research of selenium-enriched lactic acid bacteria, bifidobacteria and yeasts and influence of low-pH stress exposure on their selenium biotransformation efficiency.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
1096-0481
Volume of the periodical
105
Issue of the periodical within the volume
January 2022
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
104260
UT code for WoS article
000718040600002
EID of the result in the Scopus database
2-s2.0-85118498456