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Regulation of inflammatory pathways by cannabigerol in the collagen induced arthritis model in rats

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43933210" target="_blank" >RIV/60461373:22340/25:43933210 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11110/25:10505764 RIV/00064165:_____/25:10505764

  • Result on the web

    <a href="https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1705962/full" target="_blank" >https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1705962/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fphar.2025.1705962" target="_blank" >10.3389/fphar.2025.1705962</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Regulation of inflammatory pathways by cannabigerol in the collagen induced arthritis model in rats

  • Original language description

    Objectives This study aims to assess the anti-inflammatory properties of cannabigerol (CBG) in collagen-induced arthritis (CIA) model in rats, and to determine which inflammatory signaling pathways it affects.Study design Rats were randomized into four groups: placebo (PCB)-p.o. Treated with 1 mL of 0.9% saline once daily, CBG-p.o. Treated with 30 mg of CBG/day, glucocorticoids (GC)-p.o. Treated with methylprednisolone 0.5 mg/kg/day, and negative control (CO)-p.o. Treated with 1 mL of 0.9% saline once daily. CIA was induced in the PCB, GC, and CBG groups. The effect of CBG was assessed by clinical scoring, paw width measurements, ELISA, and analysis of gene (qPCR) and protein (Western blot) expression of selected inflammatory markers in blood and synovial membrane.Results Clinical scores showed significant improvement in the CBG vs. PCB on day 29 and in the GC vs. PCB on days 24, 27, and 29. MMP-3 levels in serum were significantly reduced in the GC vs. PCB. CBG demonstrated a selective anti-inflammatory and immunomodulatory profile, notably through the downregulation of key signaling molecules such as TLRs, systemic NF-kappa B p65, STAT-3, and inflammasome-related components including NLRP1A, NLRP3, AIM2, gasdermin D, and caspase-1. It also reduced IL-1 beta and TNF expression during the early phase of disease and increased expression of the anti-apoptotic gene BCL-2.Conclusion Our findings indicate that CBG modulates distinct components of the inflammatory signaling pathways, and its effects translated into significant improvement in clinical scoring based on swelling, erythema, stiffness in rat CIA model.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30100 - Basic medicine

Result continuities

  • Project

    <a href="/en/project/NU22-08-00346" target="_blank" >NU22-08-00346: Combination of new cannabinoids and advanced formulation methods for treatment of rheumatoid arthritis</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Pharmacology

  • ISSN

    1663-9812

  • e-ISSN

    1663-9812

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    1705962

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    18

  • Pages from-to

    nestránkováno

  • UT code for WoS article

    001611244500001

  • EID of the result in the Scopus database

    2-s2.0-105021524653