Investigating drug-liposome interactions using liposomal electrokinetic chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43933691" target="_blank" >RIV/60461373:22340/25:43933691 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/25:10511473

Result on the web

<a href="http://10.1007/s00216-025-05783-6" target="_blank" >http://10.1007/s00216-025-05783-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-025-05783-6" target="_blank" >10.1007/s00216-025-05783-6</a>

Result language

angličtina

Original language name

Investigating drug-liposome interactions using liposomal electrokinetic chromatography

Original language description

This study explores the potential of using liposomal electrokinetic chromatography as a ranking method for the rapid and simultaneous evaluation of drug-membrane interactions of a larger group of substances and assessing their sensitivity to tissue-specific parameters, namely pH, temperature, and lipid composition. We used a group of nine model drug substances to manifest how molecules could be classified for the relative sensitivity of drug-membrane interactions to pH and temperature. We observed that increasing the amount of liposomes in the background electrolyte significantly affected the separation kinetics of various active pharmaceutical ingredients, altering their mobility and/or peak shapes. Experiments with liposomes from bovine liver and heart tissue extracts revealed different interactions based on the lipid composition. Canagliflozin, which initially showed no electrophoretic mobility, migrated toward the anode in the presence of negatively charged liposomes. Mobility of positively charged substances, ambroxol and maraviroc, was suppressed by the interactions with liposomes. Their peaks also exhibited significant tailing. The effect on the separation of negatively charged compounds was significantly weaker. A small change in mobility was observed only in the case of deferasirox. We also examined the effect of temperature during separation, and we observed that increased temperature generally enhanced effective mobility due to lower electrolyte viscosity and increased lipid bilayer fluidity. Lastly, we tested the effect of sodium phosphate buffer pH (ranging from 6.0 to 8.0) with 4% liposomes on drug-liposome interactions. However, the effects were complex due to changes in API ionization and liposome surface charge, complicating the distinction between pH effects and liposome presence on API behavior. Our findings emphasize the significance of liposome composition, temperature, and pH in studying the interactions of liposomes with drugs, which is crucial for optimizing liposome-based drug delivery systems.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/GA24-11986S" target="_blank" >GA24-11986S: Liposomal probes into colligative drug permeability</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ISSN

1618-2642

e-ISSN

1618-2650

Volume of the periodical

417

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

2029-2038

UT code for WoS article

001418893100001

EID of the result in the Scopus database

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