Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F20%3A00000498" target="_blank" >RIV/61383082:_____/20:00000498 - isvavai.cz</a>
Alternative codes found
RIV/27283933:_____/19:00007310 RIV/65269705:_____/19:00070951
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/trf.15170" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/trf.15170</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/trf.15170" target="_blank" >10.1111/trf.15170</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method
Original language description
BACKGROUND: Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. METHODS: Plasma-containing products from group A and O donors were titered using the participant's routine IS one-dilution titer method. No time or temperature incubations were performed, and antihuman globulin reagent was not used. The samples were then tested using a reference method, which was a saline tube test with a 1-hour room temperature incubation; antihuman globulin was not used in the reference method. The results of the one-dilution titer were then compared to that obtained in the reference method. RESULTS: Nine centers participated in this study. There were 698 antibodies from 374 units tested by the manual IS tube one-dilution titer method; sensitivity was 0.88 (95% confidence interval [CI], 0.83–0.92), and specificity was 1.00 (95% CI, 0.98–1.00). There were 412 antibodies from 206 units tested by the manual and automated IS buffered gel card one-dilution titer method; sensitivity was 0.95 (95% CI, 0.91–0.98), and specificity was 0.87 (95% CI, 0.81–0.91). There were 98 antibodies from 49 units tested by an automated microplate IS one-dilution titer method; sensitivity was 0.76 (95% CI, 0.71–0.93), and specificity was 0.96 (95% CI, 0.92–0.99). All three methods had an accuracy rate of 90% or greater. CONCLUSION: The manual and automated one-dilution titer methods are suitable for screening plasma-containing units, although more evaluation of the automated microplate method might be required.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Transfusion
ISSN
0041-1132
e-ISSN
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Volume of the periodical
59
Issue of the periodical within the volume
Suppl 2
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
1512-1517
UT code for WoS article
000469482400015
EID of the result in the Scopus database
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