Membrane Binding of Peripheral Proteins and its Characterisation Applying Fluorescence Methods.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F02%3A54030097" target="_blank" >RIV/61388955:_____/02:54030097 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Membrane Binding of Peripheral Proteins and its Characterisation Applying Fluorescence Methods.
Original language description
Basically, the problem of membrane binding of peripheral proteins can be studied in two ways. On the one hand we can address the protein itself, using either intrinsic tryptophan fluorescence or dyelabelled protein, as typically employed in fluorescencerecovery after photobleaching experiments. On the other hand we can approach the binding event from the membrane side, studying solvent relaxation, fluorescence anisotropy or excimer formation of suitable membrane probes. For the case of the blood coagulation protein prothrombin and its fragment l the combined use of these fluorescence methods shows a slightly tighter binding of the fragment l portion in the case of the entire prothrombin molecule compared to the isolated fragment l while no evidence could be obtained for hydrophobic membrane binding sites in the ''non-fragment l'' part of prothrombin.
Czech name
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Czech description
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Project
<a href="/en/project/LN00A032" target="_blank" >LN00A032: Structure and dynamics of complex molecular systems and biomolecules</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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