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Voltametric Determination of Adenine, Guanine and DNA Using Liquid Mercury Free Polished Silver Solid Amalgam Electrode

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F04%3A00101047" target="_blank" >RIV/61388955:_____/04:00101047 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Voltametric Determination of Adenine, Guanine and DNA Using Liquid Mercury Free Polished Silver Solid Amalgam Electrode

  • Original language description

    Liquid mercury free polished silver solid amalgam electrode (p-AgSAE) proved experimentally as suitable substitute for the hanging mercury drop electrode in cathodic stripping voltammetric analyses of purine bases and of acid treated DNA. The analyses are done in alkaline solution in the presence of copper(II) ions. With p-AgSAE using direct current voltammetry the limits of detection for purine bases as well as for two types of DNA were on nanomol level. Low levels of RSD (1-3 %, n = 11) of repeated measurements confirmed that the used mode of electrochemical regeneration of p-AgSAE was sufficient, and it provided reliable measurement results. The mentioned working electrode is suitable for a sensitive electrochemical detection of acid-hydrolyzed DNA,and may be applied in DNA hybridization assays as an alternative to working electrodes based on liquid mercury (HMDE, meniscus AgSAE).

  • Czech name

    Voltametrické stanovení adeninu, guaninu a DNA za použití leštěné stříbrné amalgámové elektrody

  • Czech description

    Leštěná stříbrná amalgámova elektroda (p-AgSAE), neobsahující kapalnou rtuť, se experimentálně prokázala jako vhodná náhradu visicí rtuťové kapkové elektrody pro katodickou rozpouštěcí voltametrickou analýzu purinových bází a DNA. Analýza se provádí v alkalickém roztoku za přítomnosti Cu(II) iontů. S p-AgSAE za použití DC voltametrie byla dosažena mez detekce purinových bází na hladině nanomolární. Tato elektroda se jeví jako vhodná pro citlivou elektrochemickou detekci kyselého hydrolyzátu DNA a může být aplikována v testech hybridizace DNA jako alternativa k pracovní elektrodě založení na rtuti (HMDE, meniskové AgSAE).

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CG - Electrochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/KSK4040110" target="_blank" >KSK4040110: Theoretical fundamentals of chemical reactions and processes</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Letters

  • ISSN

    0003-2719

  • e-ISSN

  • Volume of the periodical

    37

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    399-413

  • UT code for WoS article

  • EID of the result in the Scopus database