Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F11%3A00358473" target="_blank" >RIV/61388955:_____/11:00358473 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00216-011-4694-z" target="_blank" >http://dx.doi.org/10.1007/s00216-011-4694-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-011-4694-z" target="_blank" >10.1007/s00216-011-4694-z</a>
Alternative languages
Result language
angličtina
Original language name
Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS
Original language description
Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
399
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
3547-3554
UT code for WoS article
000288398700025
EID of the result in the Scopus database
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