Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F12%3A00381241" target="_blank" >RIV/61388955:_____/12:00381241 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/C2CP41275F" target="_blank" >http://dx.doi.org/10.1039/C2CP41275F</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/C2CP41275F" target="_blank" >10.1039/C2CP41275F</a>

Result language
angličtina
Original language name
Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
Original language description
Although there were experimental indications that phospholipid bilayers hydrated with D2O express different biophysical properties compared with hydration by ordinary H2O, a molecular concept for this behavior difference was only recently proposed by a molecular dynamics simulations study [T. Róg et al., J. Phys. Chem. B, 2009, 113, 2378?2387]. Here we attempt to verify those theoretical predictions by fluorescence measurements on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. Specifically, we determine the water isotope effect on headgroup hydration and mobility, lateral lipid diffusion and lipid backbone packing. Time-dependent fluorescence shift experiments show significantlyslower dynamics and lower hydration of the headgroup region for a bilayer hydrated with D2O, an observation in good agreement with the calculated predicted differences in duration of lipid?lipid and lipid?water bridges and extent of wate
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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