FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F13%3A00391710" target="_blank" >RIV/61388955:_____/13:00391710 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/13:00391710
Result on the web
<a href="http://dx.doi.org/10.1016/j.chemphyslip.2013.02.006" target="_blank" >http://dx.doi.org/10.1016/j.chemphyslip.2013.02.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemphyslip.2013.02.006" target="_blank" >10.1016/j.chemphyslip.2013.02.006</a>
Alternative languages
Result language
angličtina
Original language name
FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion
Original language description
The observation that 22-NBD-cholesterol appears to be indifferent to the changes in the membrane packing in living cells is in agreement with previous studies in model membranes. However, our data indicate that the alternation of plasma membrane structure induced by decrease of cholesterol level by beta-CDX makes the membrane environment of NBD moiety of 25-NBD-cholesterol probe a significantly more hydrated. This finding not only encourages using 25-NBD-cholesterol in living cells, but also demonstrates that previously drawn discouraging conclusions on the use of 25-NBD-cholesterol in model membranes are not valid for living cells
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP207%2F12%2F0919" target="_blank" >GAP207/12/0919: The role of hydrophobic plasma membrane interior in DOR regulation of trimeric G protein activity</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry and Physics of Lipids
ISSN
0009-3084
e-ISSN
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Volume of the periodical
167-168
Issue of the periodical within the volume
FEB-MAR
Country of publishing house
IE - IRELAND
Number of pages
8
Pages from-to
62-69
UT code for WoS article
000318137600008
EID of the result in the Scopus database
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