Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F13%3A00392185" target="_blank" >RIV/61388955:_____/13:00392185 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2013.03.055" target="_blank" >http://dx.doi.org/10.1016/j.aca.2013.03.055</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2013.03.055" target="_blank" >10.1016/j.aca.2013.03.055</a>

Result language
angličtina
Original language name
Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam
Original language description
A flow amperometric enzymatic biosensor for the determination of glucose was constructed. The biosensor consists of a flow reactor based on porous silver solid amalgam (AgSA) and a flow tubular detector based on compact AgSA. The preparation of the sensor and the determination of glucose occurred in three steps. First, a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed at the porous surface of the reactor. Second, enzyme glucose oxidase (GOx) was covalently immobilized at MUA-layer using N-ethyl-N-(3-dimethylaminopropyl) carboimide and N-hydroxysuccinimide chemistry. Finally, a decrease of oxygen concentration (directly proportional to the concentration of glucose) during enzymatic reaction was amperometrically measured on the tubular detector under flow injection conditions. The following parameters of glucose determination were optimized with respect to amperometric response: composition of the mobile phase, its concentration, the potential of detection and the
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F11%2F1638" target="_blank" >GAP206/11/1638: Novel electrochemical sensors and sensing techniques for the analysis of nucleic acids structure and interactions</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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