Adsorption of papain on solid substrates of different hydrophobicity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00462171" target="_blank" >RIV/61388955:_____/16:00462171 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1116/1.4958305" target="_blank" >http://dx.doi.org/10.1116/1.4958305</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1116/1.4958305" target="_blank" >10.1116/1.4958305</a>

Result language

angličtina

Original language name

Adsorption of papain on solid substrates of different hydrophobicity

Original language description

Adsorption properties of protein Papain at the solidjliquid (0.1M KCl) interfaces of different hydrophobicity [highly oriented pyrolytic graphite (HOPG), bare gold, CH3, OH, and COOHterminated self-assembled monolayers on gold] were studied by a combined quartz crystal microbalance and atomic force microscopy techniques. It was found that Papain forms an incomplete monolayer at hydrophobic interfaces (HOPG and CH3-terminated substrate), whereas on more hydrophilic ones, a complete monolayer formation was always observed with either the onset of the formation of a second layer (bare gold substrate) or adsorption in a multilayer fashion, possibly a bilayer formation (OH-terminated substrate). The surface concentration and compact monolayer film thickness was much lower on the COOH-terminated substrate compared to other surfaces studied. This result was explained by partial dissociation of the interfacial COOH groups leading to additional electrostatic interactions between the positively charged protein domains and negatively charged carboxylate anions, as well as to local pH changes promoting protein denaturation. © 2016 American Vacuum Society.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biointerphases

ISSN

1934-8630

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

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UT code for WoS article

000386586100004

EID of the result in the Scopus database

2-s2.0-84978997917