Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00490126" target="_blank" >RIV/61388955:_____/18:00490126 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.026" target="_blank" >http://dx.doi.org/10.1016/j.jelechem.2018.03.026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.026" target="_blank" >10.1016/j.jelechem.2018.03.026</a>
Alternative languages
Result language
angličtina
Original language name
Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study
Original language description
The ability to tune the permeability of cell membranes might have significant implications in targeted drug delivery applications. In this work we employ electrochemical methods as well as nanolithography realized in the environment of in-situ atomic force microscopy (AFM) to demonstrate that bioactive compound apigenin is capable of altering the permeability of a model phospholipid dipalmitoylphosphatidylcholine bilayer supported by a gold surface. The electron transfer rate through the bilayer was investigated employing [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox couple as an electroactive probe. The value of the standard heterogeneous rate constant obtained in the absence of apigenin (k 0 = 6.60∙10 −5 cm·s −1 ) indicates that the electron transfer through the bilayer is rather sluggish confirming its compactness. The addition of apigenin to the system leads to a considerable increase of the electron transfer rate to k 0 = 5.74∙10 −3 cm·s −1 being virtually identical to the value obtained at the bare electrode/electrolyte interface (k 0 = 2.24∙10 −3 cm·s −1 ). This suggests that apigenin induces a significant rearrangement of the supported lipid bilayer increasing thus its permeability. The AFM nanolithography performed in-situ in the presence of apigenin confirmed that the supported lipid bilayer reorganizes itself to a monolayer of molecules laying parallel to the electrode surface.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Electroanalytical Chemistry
ISSN
1572-6657
e-ISSN
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Volume of the periodical
821
Issue of the periodical within the volume
JUL 2018
Country of publishing house
CH - SWITZERLAND
Number of pages
6
Pages from-to
67-72
UT code for WoS article
000437818600011
EID of the result in the Scopus database
2-s2.0-85044052308