Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00498461" target="_blank" >RIV/61388955:_____/18:00498461 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/18:00498461
Result on the web
<a href="https://www.pnas.org/content/115/47/11923" target="_blank" >https://www.pnas.org/content/115/47/11923</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1811520115" target="_blank" >10.1073/pnas.1811520115</a>
Alternative languages
Result language
angličtina
Original language name
Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore
Original language description
Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid membrane, they instead passively enter vesicles and live cells by inducing membrane multilamellarity and fusion. The molecular picture of this penetration mode, which differs qualitatively from the previously proposed direct mechanism, is provided by molecular dynamics simulations. The kinetics of vesicle agglomeration and fusion by an iconic cell-penetrating peptide-nonaarginine-are documented via real-time fluorescence techniques, while the induction of multilamellar phases in vesicles and live cells is demonstrated by a combination of electron and fluorescence microscopies. This concert of experiments and simulations reveals that the identified passive cell penetration mechanism bears analogy to vesicle fusion induced by calcium ions, indicating that the two processes may share a common mechanistic origin.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
115
Issue of the periodical within the volume
47
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
11923-11928
UT code for WoS article
000450642800040
EID of the result in the Scopus database
2-s2.0-85056778564