The impact of lipid oxidation on the functioning of a lung surfactant model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00506534" target="_blank" >RIV/61388955:_____/18:00506534 - isvavai.cz</a>

Result on the web

<a href="http://hdl.handle.net/11104/0297761" target="_blank" >http://hdl.handle.net/11104/0297761</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c8cp04496a" target="_blank" >10.1039/c8cp04496a</a>

Result language

angličtina

Original language name

The impact of lipid oxidation on the functioning of a lung surfactant model

Original language description

Apart from being responsible for sufficient pulmonary compliance and preventing alveolar collapse, lung surfactant (LS) also forms the first barrier for uptake of inhaled pathogens. As such it is susceptible to damage caused by various deleterious compounds present in air, e. g. oxidants capable of oxidizing unsaturated LS lipids. This study examines the consequences of oxidizing 20% of unsaturated lipids in an LS model: a mixed 1 : 1 DPPC: POPC monolayer. POxnoPC (1-palmitoyl-2-(9-oxo-nonanoyl)-sn-glycero3- phosphocholine) is considered as the main oxidation product. Experimental surface pressure-area isotherms and polarization-modulation infrared reflection-absorption spectroscopy are employed to probe changes in the macroscopic properties of the unsaturated lipid monolayer induced by oxidation. Microscopic details of the influence of oxidation on the monolayer's phase behavior are elucidated by molecular dynamics simulations at varying lipid packing. We demonstrate that unsaturated lipid oxidation shifts the isotherm towards larger areas and advances monolayer collapse. This is caused by a reversal of the oxidized sn-2 chains of POxnoPC towards the subphase, driven by electrostatic interactions between the aldehyde, glycerin, and water. Increased lipid bulkiness, hindered transition to the LC phase, and transfer of oxidized chain terminals to the subphase have been identified as the most troublesome consequences of this process. They result in the reduction of monolayer stability and its capability to withstand high surface pressures. This may lead to uncontrolled and irreversible loss of lipids from the surface.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/GA17-06792S" target="_blank" >GA17-06792S: Lung surfactant under oxidative stress: molecular dynamics simulations and Langmuir film experiments</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physical Chemistry Chemical Physics

ISSN

1463-9076

e-ISSN

—

Volume of the periodical

20

Issue of the periodical within the volume

38

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

24968-24978

UT code for WoS article

000449171800050

EID of the result in the Scopus database

2-s2.0-85054413058