Oligomeric Architecture of Mouse Activating Nkrp1 Receptors on Living Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00505686" target="_blank" >RIV/61388955:_____/19:00505686 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/19:00505686
Result on the web
<a href="https://www.mdpi.com/1422-0067/20/8/1884" target="_blank" >https://www.mdpi.com/1422-0067/20/8/1884</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20081884" target="_blank" >10.3390/ijms20081884</a>
Alternative languages
Result language
angličtina
Original language name
Oligomeric Architecture of Mouse Activating Nkrp1 Receptors on Living Cells
Original language description
Mouse activating Nkrp1 proteins are commonly described as type II transmembrane receptors with disulfide-linked homodimeric structure. Their function and the manner in which Nkrp1 proteins of mouse strain (C57BL/6) oligomerize are still poorly understood. To assess the oligomerization state of Nkrp1 proteins, mouse activating EGFP-Nkrp1s were expressed in mammalian lymphoid cells and their oligomerization evaluated by Forster resonance energy transfer (FRET). Alternatively, Nkrp1s oligomers were detected by Western blotting to specify the ratio between monomeric and dimeric forms. We also performed structural characterization of recombinant ectodomains of activating Nkrp1 receptors. Nkrp1 isoforms c1, c2 and f were expressed prevalently as homodimers, whereas the Nkrp1a displays larger proportion of monomers on the cell surface. Cysteine-to-serine mutants revealed the importance of all stalk cysteines for protein dimerization in living cells with a major influence of cysteine at position 74 in two Nkrp1 protein isoforms. Our results represent a new insight into the oligomerization of Nkrp1 receptors on lymphoid cells, which will help to determine their function.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
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Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
8
Country of publishing house
CH - SWITZERLAND
Number of pages
24
Pages from-to
1884
UT code for WoS article
000467648700082
EID of the result in the Scopus database
2-s2.0-85065116228