Coated nanodiamonds interact with tubulin beta-III negative cells of adult brain tissue
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F20%3A00537612" target="_blank" >RIV/61388955:_____/20:00537612 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/20:00537612 RIV/68407700:21460/20:00357347
Result on the web
<a href="https://avs.scitation.org/doi/10.1116/6.0000525" target="_blank" >https://avs.scitation.org/doi/10.1116/6.0000525</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1116/6.0000525" target="_blank" >10.1116/6.0000525</a>
Alternative languages
Result language
angličtina
Original language name
Coated nanodiamonds interact with tubulin beta-III negative cells of adult brain tissue
Original language description
Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain tissue. First, we cultured neuronal tissue with ND ex vivo and studied cell prosperity, regeneration, cytokine secretion, and nanodiamond uptake. Then, we applied NDs systemically into C57BL/6 animals and assessed accumulation of nanodiamonds in brain tissue and cytokine response. We found that only non-neuronal cells internalized coated nanodiamonds and responded by excretion of interleukin-6 and interferon-gamma. Cells of neuronal origin expressing tubulin beta-III did not internalize any NDs. Once we applied coated NDs intravenously, we found no presence of NDs in the adult cortex but observed transient release of interleukin-1 alpha. We conclude that specialized adult neuronal cells do not internalize plain or coated NDs. However, coated nanodiamonds interact with non-neuronal cells present within the cortex tissue. Moreover, the coated NDs do not cross the blood-brain barrier but they interact with adjacent barrier cells and trigger a temporary cytokine response. This study represents the first report concerning interaction of NDs with adult brain tissue.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biointerphases
ISSN
1934-8630
e-ISSN
—
Volume of the periodical
15
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
32
Pages from-to
061009
UT code for WoS article
000595320700001
EID of the result in the Scopus database
2-s2.0-85097121434