Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00537129" target="_blank" >RIV/61388955:_____/21:00537129 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0314875" target="_blank" >http://hdl.handle.net/11104/0314875</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/D0FO01667E" target="_blank" >10.1039/D0FO01667E</a>
Alternative languages
Result language
angličtina
Original language name
Antitumor and antioxidant activities of purple potato ethanolic extract and its interaction with liposomes, albumin and plasmid DNA
Original language description
The aim of study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidative activity of BCE was determined in relation to liposome membranes, and peroxidation was induced by UVB and AAPH. To clarify the antioxidant activity of BCE, we investigated its interactions with hydrophilic and hydrophobic regions of a membrane using fluorimetric and FTIR methods. Next, we investigated the cytotoxicity and pro-apoptotic activities of BCE on two human colon cancer cell lines (HT-29 and Caco-2) and on normal cells (IPEC-J2 ). In addition, the ability to inhibit enzymes which are involved in pro-inflammatory reactions was examined. Furthermore, BCE interactions with serum albumin and plasmid DNA were investigated using steady state fluorescence spectroscopy and single molecule fluorescence technique (TCSPC-FCS). We proved that BCE effectively protects lipid membranes against the process of peroxidation and successfully inhibits the cyclooxygenase and lipoxygenase enzymes. What is more, it interacts with the hydrophilic and hydrophobic parts of lipid membranes as well as with albumin and plasmid DNA. It occurred, that BCE is more cytotoxic against colon cancer cell lines than normal IPEC-J2 cells, it also induces apoptosis in cancer cell lines, but does not induce cell death on normal cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GX19-26854X" target="_blank" >GX19-26854X: Concert of lipids, ions, and proteins in cell membrane dynamics and function</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food & Function
ISSN
2042-6496
e-ISSN
2042-650X
Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
20
Pages from-to
1271-1290
UT code for WoS article
000618057700026
EID of the result in the Scopus database
2-s2.0-85100912254