The role of prolines and glycine in the transmembrane domain of LAT

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00539844" target="_blank" >RIV/61388955:_____/21:00539844 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/21:00554617 RIV/00216208:11310/21:10440413
Result on the web
<a href="https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.15713" target="_blank" >https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.15713</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/febs.15713" target="_blank" >10.1111/febs.15713</a>

Result language
angličtina
Original language name
The role of prolines and glycine in the transmembrane domain of LAT
Original language description
Linker for activation in T cells (LAT) is a critical regulator of T‐cell development and function. It organises signalling events at the plasma membrane. However, the mechanism, which controls LAT localisation at the plasma membrane, is not fully understood. Here, we studied the impact of helix‐breaking amino acids, two prolines and one glycine, in the transmembrane segment on localisation and function of LAT. Using in silico analysis, confocal and super‐resolution imaging and flow cytometry, we demonstrate that central proline residue destabilises transmembrane helix by inducing a kink. The helical structure and dynamics are further regulated by glycine and another proline residue in the luminal part of LAT transmembrane domain. Replacement of these residues with aliphatic amino acids reduces LAT dependence on palmitoylation for sorting to the plasma membrane. However, surface expression of these mutants is not sufficient to recover function of nonpalmitoylated LAT in stimulated T cells. These data indicate that geometry and dynamics of LAT transmembrane segment regulate its localisation and function in immune cells.n
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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