Diffusive dynamics of bacterial proteome as a proxy of cell death
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00566839" target="_blank" >RIV/61388955:_____/23:00566839 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0338111" target="_blank" >https://hdl.handle.net/11104/0338111</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acscentsci.2c01078" target="_blank" >10.1021/acscentsci.2c01078</a>
Alternative languages
Result language
angličtina
Original language name
Diffusive dynamics of bacterial proteome as a proxy of cell death
Original language description
Temperature variations have a big impact on bacterial metabolism and death, yet an exhaustive molecular picture of these processes is still missing. For instance, whether thermal death is determined by the deterioration of the whole or a specific part of the proteome is hotly debated. Here, by monitoring the proteome dynamics of E. coli, we clearly show that only a minor fraction of the proteome unfolds at the cell death. First, we prove that the dynamical state of the E. coli proteome is an excellent proxy for temperature-dependent bacterial metabolism and death. The proteome diffusive dynamics peaks at about the bacterial optimal growth temperature, then a dramatic dynamical slowdown is observed that starts just below the cell’s death temperature. Next, we show that this slowdown is caused by the unfolding of just a small fraction of proteins that establish an entangling interprotein network, dominated by hydrophobic interactions, across the cytoplasm. Finally, the deduced progress of the proteome unfolding and its diffusive dynamics are both key to correctly reproduce the E. coli growth rate.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Central Science
ISSN
2374-7943
e-ISSN
2374-7951
Volume of the periodical
9
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
93-102
UT code for WoS article
000914868100001
EID of the result in the Scopus database
2-s2.0-85147138801