Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F24%3A00583598" target="_blank" >RIV/61388955:_____/24:00583598 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10487974
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1567539424000252?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1567539424000252?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioelechem.2024.108663" target="_blank" >10.1016/j.bioelechem.2024.108663</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.
Original language description
A new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were tested. Only covalent bonds throughout a filter-aminosilane-glutaraldehyde-enzyme chain ensured a long-time and reproducible biosensor response. The developed method of biosensor preparation has been successfully applied to enzymes glucose oxidase, laccase and choline oxidase. The dependences of peak current on detection potential, flow rate, injection volume, analyte concentration as well as biosensor lifetime and reproducibility were investigated for glucose oxidase biosensor. The sensitivity of measurements was two or more times higher than that of biosensor with a mini-reactor filled by powder with immobilized enzyme. The developed biosensor with laccase was tested by determining dopamine in the pharmaceutical infusion product Tensamin. Results of the analysis (40.0±0.7mgmL-1, SD=0.8mgmL-1, RSD=1.85%, N=11) show a good agreement with the manufacturer's declared value.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GA23-07292S" target="_blank" >GA23-07292S: Additive or subtractive manufacturing: Which is more powerful to achieve stable electroreduction of carbon dioxide at high reaction rates?</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioelectrochemistry
ISSN
1567-5394
e-ISSN
1878-562X
Volume of the periodical
157
Issue of the periodical within the volume
JUN 2024
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
108663
UT code for WoS article
001183464500001
EID of the result in the Scopus database
2-s2.0-85185194393