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Fast prototyping of hydrophobic disposable polymer support arrays for matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of proteins by atmospheric molding

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F05%3A00021525" target="_blank" >RIV/61388963:_____/05:00021525 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Fast prototyping of hydrophobic disposable polymer support arrays for matrix-assisted laser desorption/ionization-time of flight-mass spectrometry of proteins by atmospheric molding

  • Original language description

    A protocol for prototyping hydrophobic disposable copolymer sample support arrays for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of proteins by atmospheric molding is introduced. The one-step modification during the fabrication is demonstrated for enhanced protein adsorption to the modified materials by introduction of hydrophobic butyl-, dodecyl-, and octadecyl groups to the polymer backbone. The protein adsorption to the new hydrophobic copolymer chips was studied using different proteins.

  • Czech name

    Rychlá příprava prototypu polymerních MALDI-TOF terčíků pro analýzu proteinů s použitím atmosferického formování

  • Czech description

    Byl vypracován protokol pro přípravu hydrofobního polymerového nosiče pro hmotnostni spektrometrii s laserovou desorpci/ionisaci za účasti matrice (MALDI) a analyzátorem doby letu pomocí atmosferického formovani. Zavedením hydrofobních skupin butyl-, dodecyl-, a octadecyl na polymer bylo dosaženo zvýšení adsorpce proteinů na nosič. Adsorpce proteinů na nosič a jejich detekce hmotovou spektrometrií byly testovány s řadou proteinů.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    14

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    2835-2842

  • UT code for WoS article

  • EID of the result in the Scopus database